Fig. 4

Sample-shared SSB and AP site hotspots found within ± 200 bp of TSSs tend to associate with highly expressed genes. The box plots of the expression levels (log₂(TPM + 1), Y-axes) of genes with and without sample-shared (depth ≥ 1) AP sites (top) or SSB hotspots (bottom) within ± 200 bp of their TSSs on either template or nontemplate strands are shown for each tissue (X-axes) for AP sites and SSBs. Box plots indicate median (middle line), 25th, 75th percentile (box) and 1.5× interquartile range (whiskers) as well as outliers (single points). The asterisks above the horizontal lines denote the significance of the difference: *, **, ***, **** represent respectively p-values < 0.05, 0.01, 0.001, 0.0001 (Wilcoxon Rank Sum Test), while “ns” mean no significant difference. See Supplemental Tables 6 and Methods for more details