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Fig. 5 | BMC Genomics

Fig. 5

From: Split Pool Ligation-based Single-cell Transcriptome sequencing (SPLiT-seq) data processing pipeline comparison

Fig. 5

UMAP of data generated by each pipeline colored by predicted cell-type after using the Azimuth cell annotation algorithm. A Cell-type annotation for cells generated by alevin-fry B Cell-type annotation for cells generated by splitpipe C. Cell-type annotation for cells generated by STARsolo. Cells are color coded (also indicated at the right of Fig. 5C) by cell-type after using the Azimuth cell annotation algorithm. D An Upset plot of the predicted cell-types from the PBMC data generated by each pipeline. Set size equals the total amount of predicted cell-types per pipeline. Intersection size representing the total amount of predicted cell-types that overlap between the intersected set of pipelines. The bottom right panel shows which pipelines are being intersected. E A barplot comparing the number of cells that are predicted to be a specific cell-type per pipeline

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BMC Genomics

ISSN: 1471-2164

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