Fig. 1

Adipogenic differentiation of mesenchymal stem cells derived from different synovial tissue sources (fibrous synovium (FS) and adipose synovial tissue (FP)) of German Landrace (DL) and Angeln Saddleback (AS) breeds. Morphological changes of live cells after plating at D0 and the differentiated cells at day 1 (D1), day 4 (D4), day 7 (D7) and day 14 (D14) were presented. The lipid-droplet formation of adipocytes was observable at 4 days, and increased over time under adipogenic differentiation after culturing in an adipogenic differentiation medium. The cytoplasmic lipid-droplets deposition was observed by Oil Red-O staining on day 14 and visualized under a phase-contrast microscope (red with a clear background). Scale bars:100 μm