Fig. 7

qRT-PCR validation of adipogenic marker transcripts. Expression of adipogenic marker genes during adipogenesis at each time point compared with control groups at day 0 by qRT-PCR (A). On day 1, no significant change was observed between the control and adipo groups. On day 4, there were significant differences between control and adipo groups: CEBPA (P = 0.002), ADIPOQ (P = 0.056) and PPARG (P < 0.0001). At day 7 and 14, all adipogenic genes were significantly different at p < 0.001, except CEBPA at day 14 (p = 0.07). Differential expression of synovial mesenchymal stem cells (SMSCs) transcripts derived from German Landrace (DL) and Angeln Saddleback (AS) confirmed by qRT-PCR (B). Least squares means and standard errors were used and then transformed to relative expression levels. *P < 0.05, **P < 0.01 and ***P < 0.001