Figure 3
From: Sequencing strategy for the whole mitochondrial genome resulting in high quality sequences
Sequence analysis of different mtDNA dilutions. left: raw data and (right) electropherograms of the sequencing reactions: amplification was carried out using 1000, 2500, 5000 and 10000 genome equivalents (GEs) of mtDNA using 2 μL of purified (Microcon) PCR products for cycle sequencing. Sequence electropherograms of fragment A from position 5798 to position 5828 are shown as example. Sequences of amplicons purified with ExoSAP-IT are not displayed as they equal with respect to the quality of the purified amplicons using filtration.