- Methodology article
- Open Access
Sequencing strategy for the whole mitochondrial genome resulting in high quality sequences
© Fendt et al; licensee BioMed Central Ltd. 2009
- Received: 17 October 2008
- Accepted: 30 March 2009
- Published: 30 March 2009
It has been demonstrated that a reliable and fail-safe sequencing strategy is mandatory for high-quality analysis of mitochondrial (mt) DNA, as the sequencing and base-calling process is prone to error. Here, we present a high quality, reliable and easy handling manual procedure for the sequencing of full mt genomes that is also appropriate for laboratories where fully automated processes are not available.
We amplified whole mitochondrial genomes as two overlapping PCR-fragments comprising each about 8500 bases in length. We developed a set of 96 primers that can be applied to a (manual) 96 well-based technology, which resulted in at least double strand sequence coverage of the entire coding region (codR).
This elaborated sequencing strategy is straightforward and allows for an unambiguous sequence analysis and interpretation including sometimes challenging phenomena such as point and length heteroplasmy that are relevant for the investigation of forensic and clinical samples.
- Control Region
- Reactive Oxidative Species
- High Quality Sequence
- Length Heteroplasmy
- Control Region Haplotype
Investigations of the human mt genome are in the focus of biological and medical scientific disciplines. Compared to nuclear DNA (nDNA), mitochondrial DNA (mtDNA) is more vulnerable to oxidative damage and undergoes a higher rate of mutation . Because of these features the analysis of the mt genome has become a proven tool in population genetics. A multi-copy genome without recombination which accumulates mutations allows for the establishment of phylogenetic trees . It was the information from the highly variable mitochondrial control region (CR) that lifted the secret of human evolution starting in Africa about 150000 years ago and gave an insight in human migration all over the world within the past 60000 years [3, 4]. Sequences of full mt genomes are necessary to decipher yet not defined haplotypes and assign them to their phylogeographic environment.
Mitochondrial DNA mutations in the coding region (codR) have been associated with several pathologies  including cancer [6–9]. During oxidative phosphorylation (OXPHOS) mitochondria produce reactive oxidative species (ROS) that potentially induce DNA mutations. Such an initial mutation is heteroplasmic with the mutated variant constituting a minority . In the course of several replications the heteroplasmic mutation may become dominant leading to cancer . This theory is based on the results of several investigations on cancer tissues [11–18]. Unfortunately, numerous articles addressing that issue are erroneous as reviewed in [19, 20]. On the one hand it is the lack of phylogenetic knowledge and the ambiguous mtDNA alignment that led to false conclusions of mtDNA mutations to be tumor-specific rather than evolutionary caused. On the other hand, laboratory-, sequencing-, and analysis errors led to wrong base-calls . Hence, flawed data hamper a precise interpretation of the conjunction between mtDNA mutations and the complex process of tumor development.
For forensic as well as for phylogenetic purposes we have already successfully established evaluated sequencing strategies that proved to be useful in a number of investigations where precise base-calling was necessary for the CR [22–26], however such stringency is lacking for the whole mt genome. The published protocols vary concerning the number and sizes of PCR products, the chemistry employed, and the number of sequencing primers [27–31]. One review reports the use of 58 sets of unique sequencing primers to completely cover the mt genome, while another protocol provides 77 sequencing primers for the codR and 7 additional primers for the CR . There, sequencing is performed on 12 amplicons that cover the whole mt genome in an overlapping manner . In a recent protocol  the amplification of the entire mt genome was conducted with only two overlapping amplicons, followed by 48 upstream and downstream sequencing reactions. Whereas amplicon sizes must be kept short for forensic samples for reasons of limited DNA quality and quantity, a reduction of the necessary amplicons is desirable for other applications, where usually fresh DNA is obtained. This simplifies the laboratory work and minimizes potential amplicon mix-up . Independent of the amplification strategy high sequence quality is required to achieve reliable base-calling.
We addressed this issue by presenting a set of 96 carefully selected sequencing primers that are embedded in a reliable and fail-safe sequencing strategy. The following criteria were applied to guide the development. (1) Each nucleotide reported in the consensus sequence should derive from at least two independent sequencing reactions using different primers (double strand coverage) to avoid the reporting of phantom mutations and other ambiguous base-callings. (2) We envision a minimum number of PCR products to reduce the chance for amplicon mix-up during the (manual) set-up of sequencing reactions and (3) we selected primers that produce sequences with an optimal signal-to-noise ratio to enable unequivocal assignment of point and length heteroplasmy.
List of primers for amplification and full double-stranded sequence coverage of the entire mtDNA codR
Amplicons were purified according to two different protocols. We compared an enzymatic digestion method with a filtration method (Figure 2). We did not observe significant differences with respect to the DNA amount after purification except for one sample. There, the banding signal of the ExoSAP-IT purified product was less dense as compared to the filtrated one. However, despite of the different banding intensities (Figure 2) we did not observe relevant changes in sequencing quality between the electropherograms (Figure 3). The filtration method has the appealing advantage that it is less expensive for PCR product purification of few samples present in large volumes (up to 0.5 ml). One Microcon column is needed for each sample irrespective of the DNA volume to be added, whereas the required amount of ExoSAP-IT is required to be proportional to the volume of DNA (2 μL per 5 μL PCR-product).
Sequence electropherograms did not differ substantially in terms of the signal-to-noise ratio as well as with respect to the relative peak heights when comparing the tested mtDNA template amounts. Although a relative increase of the fluorescence signal in the raw data was detected (Figure 3, left), the quality of the sequence electropherograms were comparable (Figure 3, right). In this study we tested template mtDNA amounts between 1000 and 10000 GEs that turned out to cover an appropriate template amount range for long PCR fragments.
Data analysis and quality assurance
Upon analysis of the raw data the sequences were aligned and the base-calls reviewed twice by two independent scientists, such as has been found invaluable for CR analysis . Consensus sequences were aligned and compared to the revised Cambridge Reference Sequence (rCRS) [36, 37] following nomenclature guidelines for mtDNA typing [38–40]. In an independent analysis the two consensus sequences underwent comparison by means of a dedicated in-house software [41, 42]. This concept enabled full electronic data handling minimizing the risk to introduce clerical errors.
Assignment of the samples to their specific haplogroups
We present a reliable laboratory strategy for the amplification and sequence analysis of the full mt genome that meets a high quality demand. The method is conceived for samples that include an adequate amount and quality of DNA, such as found in fresh clinical and forensic samples and can be conducted by any laboratory in the molecular field without the requirement for automated liquid handling devices. The complexity of the laboratory concept is low, so that handling errors and risk of sample mix-up and contamination are minimized.
Effects of primer-storage on sequencing quality
It has been shown in this study that the sequence performance of the majority of primers was not affected by different storage conditions. Only 6 primers (R4, R7, R18, R20, R21, F14) suffered from a treatment of more than 5 freezing/thawing cycles. Temperature changes caused a diminished function visualized by a disadvantageous signal-to-noise ratio depicted in the electropherogram. The ability to correctly identify point heteroplasmy strongly depends on the quality of the overall signal height and the signal-to-noise ratio. Consequently, an apparent improvement of the storing conditions would be the generation of stock plates including less primer volume sufficient for about 5 sequencing reactions. Alternatively, sequencing primers could be kept in a low tris buffer at 4°C.
Applications in various fields
Recent research has been addressing the role of mitochondria and the mtDNA in aging and cancer as mitochondria participate in fundamental processes of the cellular metabolism . The mitochondrial theory of aging implies that tissue function sustains until the number of cells declines below a threshold. The time range required to reach this threshold is related to the rate at which mitochondrial and mtDNA damage accumulates. If the mitochondrial ROS production rate increases, the rate of cell loss will also increase, resulting in early tissue failure and age-related disease.
Not only aging processes but also carcinogenesis have been linked to mutation based mitochondrial dysfunction since a general feature of tumor biology is the impaired energy metabolism . For those investigations addressing aging and tumor biology, mitochondrial genes encoded in the nucleus and in the mitochondrial DNA are being analyzed. Genes encoded in the mitochondrial DNA can be systematically targeted with this evaluated sequencing strategy.
Furthermore, it can be helpful for population genetic and forensic applications where further information to the CR is required. In the forensic context this is particularly relevant for the few CR haplotypes that are shared more commonly within a population. In the West Eurasian population this is the case for the CR haplotypes 16519C 263G 315.1C and 263G 315.1C, which occur at frequencies of 0.013 and 0.002, respectively . When such haplotypes are observed in a forensic case between suspect and crime scene samples one needs to consider the possibility that the sequences are identical by state and not by descent. Especially, as it is known that these haplotypes occur in more haplogroup backgrounds, such as H1, H2a, H3, H7, H10, H13a1, HV, R0 and the great bulk of yet unidentified sub-H lineages [26, 49]. To increase the information content of such haplotypes we have earlier introduced a screening method based on single-base-extension reactions that target informative SNPs (single nucleotide polymorphism) in the codR . Such screening methods are a valuable supplement to standard mtDNA sequencing as they generally increase the discrimination power and indicate the hg-affiliation of an mtDNA haplotype . The logical continuation of this strategy would be the analysis of entire mt genomes in forensic casework for achieving maximum discrimination power. The current sequencing technology allows that only for relatively high sample qualities, but it is a first step towards mt genome sequencing in forensics.
In the population genetic field an accurate deciphering of the human mitochondrial phylogeny can only be conducted on the basis of full mt genomes. As a general approach full genome mtDNA sequencing is carried out on a defined selection of CR sequences as evidenced by recent examples [51, 52].
Meaning of obtaining high quality sequences
The focus of this strategy lies on applications where secure base-calling and high quality sequence data are mandatory for interpretation. It is well known that mitochondrial mutations associated to disease such as mitochondrial encephalomyopathies occur in heteroplasmic status and that the severity and the progression of the syndrome depend on a threshold above which the mutant triggers the pathological pathway [53, 54]. The contribution of mtDNA mutations to carcinogenesis underlies the same assumption. It appears evident that particular mitochondrial defects with functional consequences exhibit an advantage in tumor development only if a certain threshold of mutated mtDNA populations is achieved. All the more it is important to pinpoint the relative quantity of these heteroplasmic mutations to estimate functional consequences of the genes involved. Moreover, good sequencing quality is the prerequisite to distinguish early stage point heteroplasmy from signal background which might be an issue in early stage cancer detection .
The occurrence and the frequency of mtDNA control region (CR) point heteroplasmy is an important issue in forensic case work  as the detection of point heteroplasmy, especially at low level, increases the power of discrimination between sequences .
The codR sequencing method described herein is an optimized protocol that can also be applied in laboratories that do not have automated processes available. The overall aim, namely the achievement of a secure base-calling method was accomplished by the assortment of primers that allow for full double-stranded sequence coverage of the whole mtDNA genome (in combination with previously described CR sequencing strategies). Particular care was taken on the selection of the primers based on low background signal that is crucial for the unambiguous assessment of length and point heteroplasmy. Moreover, we kept the complexity of the laboratory process as low as possible. This was achieved by a 96- well based pipetting format for cycle sequencing set-up using only two PCR amplicons per sample which reduces the chances of contamination, handling error and sample mix-up.
A total of 10 biopsy samples were collected from patients with diagnosed invasive mamma carcinoma at the Clinical Department of Radiology, Innsbruck Medical University. They were immediately transferred into 1.5 mL vials and extracted or frozen at -20°C and extracted within the next 7 days. Corresponding blood samples were collected independently. Peripheral blood samples were either extracted upon receipt or frozen at -20°C and then processed within 7 days. There was full consent of the patients to participate in this study. For the sensitivity study DNA was extracted from peripheral blood of a healthy volunteer of European ancestry with informed consent.
The complete workflow is sketched in Figure 1. DNA was extracted from biopsies and blood via the EZ1 Biorobot workstation (Qiagen, Hilden, Germany). Extraction was based on the protocol of the investigator kit (Qiagen) for extraction of blood and tissue samples, with modifications as follows: tissue samples (about 0.5 cm × 1 mm) were digested with 15 μL proteinase K in 290 μL lysis buffer (included in the kit) up to 4 hours and treated with the "trace" protocol.
A total of 96 primers were used for singleplex reactions. Primer sequences were taken from [29, 33] and new primers were designed in this study with PRIMER 3 software . Possible heteroduplex formations, primer dimers and hairpin structures were analyzed with OligoAnalyzer free software: http://eu.idtdna.com/analyzer/Applications/OligoAnalyzer/ (Integrated DNA Technologies, Coralville, IA). Melting temperature of primers was approximately 50°C and the GC-content was between 40 – 60%. The settings for the calculations were: Oligo conc. n: 0.25 μM; Na+ conc.: 50 mM.
Amplification and Sequencing
For amplification we used the Advantage GC Genomic LA Polymerase(Clontech, Bella Avenue Mountain View, California) including a small amount of proofreading enzyme, a hot start antibody and a 3' to 5' proofreading exonuclease activity. It enables synthesis of PCR products of 8.5 kb using human genomic DNA templates.
Amplification reaction was carried out on a thermal cycler (Multicycler PTC240 Tetrad2, Hercules, CA) in a total volume of 50 μL each fragment (A and B) comprising 2.5 mg/mL BSA (St. Louis, Missouri), 2.5 mM each dNTP (Applied Biosystems), 10 mM each primer, 5U LA Genomic DNA polymerase (Clonetech) 1000 – 10000 mtDNA GEs. Thermal cycling conditions comprised an initial 3 min denaturation step at 93°C, followed by 93°C for 15 s, 60°C for 30 s and 68°C for 5 min 14 times proceeding 27 times with 93°C for 15 s, 55°C for 30 s and 68°C for 9 min increasing for 10 s each cycle. Amplicons were purified from residual primers and dNTPs enzymatically with ExoSAP-IT (ExoSAP-IT, USB, Cleveland, Ohio) and comparatively with a filtration method (Microcon YM-30 Centrifugal Filter Units: Millipore, Billerica, Massachusetts).
From a 1.5 μM stock plate containing all 96 sequencing primers (Figure 1, step 4), 2 μL were decanted into a new plate right before each sequencing reaction. Big Dye Terminator mastermix (containing 1 μL BigDye Terminator v1.1 Cycle Sequencing mix (Applied Biosystems) and 3 μL BigDye Terminator v1.1 Sequencing Buffer (Applied Biosystems)), as well as 2 μL mtDNA Fragments A or B were aliquoted into the appropriate wells as shown in Figure 1 (step 4). 2 μL distilled water was added to reach a final volume of 10 μL. Pipetting those small volumes was performed using an 8-channel epMotion workstation (Eppendorf AG, Hamburg, Germany). Cycle sequencing was performed (after a first denaturation step of 95°C for 1 min) for 25 cycles of 10 s at 95°C, 5 s at 50°C, and 4 min at 60°C.
Post sequencing cleanup
Sequencing reaction products were purified from residual dye terminators using Sephadex G-50 Fine (Amersham, Buckinghamshire, United Kingdom) and Multiscreen filter plates (Millipore) according to the manufacturer's protocol. The cycle sequencing products were diluted by adding 10 μL of distilled water and the dilutions were centrifuged through the filter plate into an optical 96-well plate for electrophoretic separation. The entire procedure of diluting cycle sequencing products and transferring the dilutions onto the Sephadex columns in the filter plate was again performed by the epMotion workstation. When spinning cycle sequencing products through the filter plate, unequal amounts of product may be recovered throughout the plate. In order to avoid this, the blocks' orientations in the centrifuge carriage were reversed after 2.5 min and the blocks were spun a second time for 2.5 min to obtain consistent amounts of purified products. The purified products were finally diluted by adding each 20 μL of distilled water to achieve volumes of 45 μL.
Electrophoretic separation was carried out on an ABI 3100 capillary sequencer (Applied Biosystems) using POP6 and a 36 cm capillary array. The run module conditions were as follows: injection time: 22 s, injection voltage: 1 kV, run voltage: 15 kV, run current: 10 μAmps, run temperature: 55°C.
The authors would like to thank Birgit Krabichler (Sektion für Humangenetik, Medizinische Universität Innsbruck) and Martin Bodner (Institut für Gerichtliche Medizin, Medizinische Universität Innsbruck) for valuable technical assistance and discussion. We would further like to acknowledge three anonymous reviewers for helpful suggestions. This research received support from the „Österreichische Forschungsförderungsgesellschaft, KIRAS Sicherheitsforschung DNATOX (813786/12828-GLE/SHJ)” and from the FWF Austrian Science Fund (TR-L397).
- Mollnau H, Wenzel P, Oelze M, Treiber N, Pautz A, Schulz E, Schuhmacher S, Reifenberg K, Stalleicken D, Scharffetter-Kochanek K, Kleinert H, Münzel T, Daiber A: Mitochondrial oxidative stress and nitrate tolerance – comparison of nitroglycerin and pentaerithrityl tetranitrate in Mn-SOD+/- mice. BMC Cardiovasc Disord. 2006, 6: 44-PubMed CentralView ArticlePubMedGoogle Scholar
- Kivisild T, Metspalu M, Bandelt HJ, Richards M, Villems R: The world of mitochondrial DNA phylogeny. Human mitochondrial DNA and the Evolution of Homo sapiens. Edited by: Bandelt HJ, Macaulay V, Richards M. 2006, Heidelberg, Springer Verlag, 149-179.Google Scholar
- Cann RL, Stoneking M, Wilson AC: Mitochondrial DNA and human evolution. Nature. 1987, 325: 31-36.View ArticlePubMedGoogle Scholar
- Torroni A, Achilli A, Macaulay V, Richards M, Bandelt HJ: Harvesting the fruit of the human mtDNA tree. Trends Genet. 2006, 22: 339-345.View ArticlePubMedGoogle Scholar
- DiMauro S, Schon EA: Mitochondrial respiratory-chain diseases. N Engl J Med. 2003, 348: 2656-2668.View ArticlePubMedGoogle Scholar
- Abnet CC, Huppi K, Carrera A, Armistead D, McKenney K, Hu N, Tang ZZ, Taylor PR, Dawsey SM: Control region mutations and the 'common deletion' are frequent in the mitochondrial DNA of patients with esophageal squamous cell carcinoma. BMC Cancer. 2004, 4: 30-PubMed CentralView ArticlePubMedGoogle Scholar
- Aikhionbare FO, Mehrabi S, Kumaresan K, Zavareh M, Olatinwo M, Odunsi K, Partridge E: Mitochondrial DNA sequence variants in epithelial ovarian tumor subtypes and stages. J Carcinog. 2007, 6: 1-PubMed CentralView ArticlePubMedGoogle Scholar
- Carew JS, Huang P: Mitochondrial defects in cancer. Mol Cancer. 2002, 1: 9-PubMed CentralView ArticlePubMedGoogle Scholar
- Cavalli LR, Liang BC: Mutagenesis, tumorigenicity, and apoptosis: are the mitochondria involved?. Mutat Res. 1998, 398: 19-26.View ArticlePubMedGoogle Scholar
- Fan W, Waymire KG, Narula N, Li P, Rocher C, Coskun PE, Vannan MA, Narula J, Macgregor GR, Wallace DC: A mouse model of mitochondrial disease reveals germline selection against severe mtDNA mutations. Science. 2008, 319: 958-962.PubMed CentralView ArticlePubMedGoogle Scholar
- Czarnecka AM, Golik P, Bartnik E: Mitochondrial DNA mutations in human neoplasia. J Appl Genet. 2006, 47: 67-78.View ArticlePubMedGoogle Scholar
- Penta JS, Johnson FM, Wachsman JT, Copeland WC: Mitochondrial DNA in human malignancy. Mutat Res. 2001, 488: 119-133.View ArticlePubMedGoogle Scholar
- Polyak K, Li Y, Zhu H, Lengauer C, Willson JK, Markowitz SD, Trush MA, Kinzler KW, Vogelstein B: Somatic mutations of the mitochondrial genome in human colorectal tumours. Nat Genet. 1998, 20: 291-293.View ArticlePubMedGoogle Scholar
- Allalunis-Turner J, Ma I, Hanson J, Pearcey RG: mtDNA mutations in invasive cervix tumors: a retrospective analysis. Cancer Lett. 2006, 243: 193-201.View ArticlePubMedGoogle Scholar
- Jeronimo C, Nomoto S, Caballero OL, Usadel H, Henrique R, Varzim G, Oliveira J, Lopes C, Fliss MS, Sidransky D: Mitochondrial mutations in early stage prostate cancer and bodily fluids. Oncogene. 2001, 20: 5195-5198.View ArticlePubMedGoogle Scholar
- Chatterjee A, Mambo E, Sidransky D: Mitochondrial DNA mutations in human cancer. Oncogene. 2006, 25: 4663-4674.View ArticlePubMedGoogle Scholar
- Abu-Amero KK, Alzahrani AS, Zou M, Shi Y: Association of mitochondrial DNA transversion mutations with familial medullary thyroid carcinoma/multiple endocrine neoplasia type 2 syndrome. Oncogene. 2006, 25: 677-684.View ArticlePubMedGoogle Scholar
- Parrella P, Xiao Y, Fliss M, Sanchez-Cespedes M, Mazzarelli P, Rinaldi M, Nicol T, Gabrielson E, Cuomo C, Cohen D, Pandit S, Spencer M, Rabitti C, Fazio VM, Sidransky D: Detection of mitochondrial DNA mutations in primary breast cancer and fine-needle aspirates. Cancer Res. 2001, 61: 7623-7626.PubMedGoogle Scholar
- Salas A, Yao YG, Macaulay V, Vega A, Carracedo A, Bandelt HJ: A critical reassessment of the role of mitochondria in tumorigenesis. PLoS Med. 2005, 2: e296-PubMed CentralView ArticlePubMedGoogle Scholar
- Bandelt HJ, Salas A, Bravi CM: What is a 'novel' mtDNA mutation – and does 'novelty' really matter?. J Hum Genet. 2006, 51: 1073-1082.View ArticlePubMedGoogle Scholar
- Salas A, Carracedo A, Macaulay V, Richards M, Bandelt HJ: A practical guide to mitochondrial DNA error prevention in clinical, forensic, and population genetics. Biochem Biophys Res Commun. 2005, 335: 891-899.View ArticlePubMedGoogle Scholar
- Eichmann C, Parson W: 'Mitominis': multiplex PCR analysis of reduced size amplicons for compound sequence analysis of the entire mtDNA control region in highly degraded samples. Int J Legal Med. 2008, 122: 385-388.View ArticlePubMedGoogle Scholar
- Lutz-Bonengel S, Sänger T, Parson W, Müller H, Ellwart JW, Follo M, Bonengel B, Niederstätter H, Heinrich M, Schmidt U: Single lymphocytes from two healthy individuals with mitochondrial point heteroplasmy are mainly homoplasmic. Int J Legal Med. 2008, 122: 189-197.View ArticlePubMedGoogle Scholar
- Parson W: The Art of Reading Sequence Electropherograms. Ann Hum Genet. 2007, 71: 276-278.View ArticlePubMedGoogle Scholar
- Parson W, Brandstätter A, Alonso A, Brandt N, Brinkmann B, Carracedo A, Corach D, Froment O, Furac I, Grzybowski T, Hedberg K, Keyser-Tracqui C, Kupiec T, Lutz-Bonengel S, Mevag B, Ploski R, Schmitter H, Schneider P, Syndercombe-Court D, Sorensen E, Thew H, Tully G, Scheithauer R: The EDNAP mitochondrial DNA population database (EMPOP) collaborative exercises: organisation, results and perspectives. Forensic Sci Int. 2004, 139: 215-226.View ArticlePubMedGoogle Scholar
- Parson W, Dür A: EMPOP – a forensic mtDNA database. Forensic Sci Int: Genetics. 2007, 1: 88-92.View ArticleGoogle Scholar
- Levin BC, Holland KA, Hancock DK, Coble M, Parsons TJ, Kienker LJ, Williams DW, Jones M, Richie KL: Comparison of the complete mtDNA genome sequences of human cell lines – HL-60 and GM10742A – from individuals with pro-myelocytic leukemia and leber hereditary optic neuropathy, respectively, and the inclusion of HL-60 in the NIST human mitochondrial DNA standard reference material – SRM 2392-I. Mitochondrion. 2003, 2: 387-400.View ArticlePubMedGoogle Scholar
- Gonder MK, Mortensen HM, Reed FA, de Sousa A, Tishkoff SA: Whole-mtDNA genome sequence analysis of ancient African lineages. Mol Biol Evol. 2007, 24: 757-768.View ArticlePubMedGoogle Scholar
- Coble MD, Just RS, O'Callaghan JE, Letmanyi IH, Peterson CT, Irwin JA, Parsons TJ: Single nucleotide polymorphisms over the entire mtDNA genome that increase the power of forensic testing in Caucasians. Int J Legal Med. 2004, 118: 137-146.View ArticlePubMedGoogle Scholar
- Rieder MJ, Taylor SL, Tobe VO, Nickerson DA: Automating the identification of DNA variations using quality-based fluorescence re-sequencing: analysis of the human mitochondrial genome. Nucleic Acids Res. 1998, 26: 967-973.PubMed CentralView ArticlePubMedGoogle Scholar
- Taylor RW, Taylor GA, Durham SE, Turnbull DM: The determination of complete human mitochondrial DNA sequences in single cells: implications for the study of somatic mitochondrial DNA point mutations. Nucleic Acids Res. 2001, 29: e74-PubMed CentralView ArticlePubMedGoogle Scholar
- Niederstätter H, Köchl S, Grubwieser P, Pavlic M, Steinlechner M, Parson W: A modular real-time PCR concept for determining the quality and quantity of human nuclear and mitochondrial DNA. Forensic Science International: Genetics. 2007, 1: 29-34.View ArticleGoogle Scholar
- Palanichamy MG, Sun C, Agrawal S, Bandelt HJ, Kong QP, Khan F, Wang CY, Chaudhuri TK, Palla V, Zhang YP: Phylogeny of mitochondrial DNA macrohaplogroup N in India, based on complete sequencing: implications for the peopling of South Asia. Am J Hum Genet. 2004, 75: 966-978.PubMed CentralView ArticlePubMedGoogle Scholar
- Rozen S, Skaletsky HJ: Primer3 on the WWW for general users and for biologist programmers. Bioinformatics Methods and Protocols: Methods in Molecular Biology. Edited by: Krawetz S, Misener S. 2000, Totowa, Humana Press, 132: 365-386.View ArticleGoogle Scholar
- Parson W, Bandelt HJ: Extended guidelines of mtDNA typing of population data in forensic science. Forensic Sci Int: Genetics. 2007, 1: 13-19.View ArticleGoogle Scholar
- Anderson S, Bankier AT, Barrell BG, de Bruijn MH, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJ, Staden R, Young IG: Sequence and organization of the human mitochondrial genome. Nature. 1981, 290: 457-465.View ArticlePubMedGoogle Scholar
- Andrews RM, Kubacka I, Chinnery PF, Lightowlers RN, Turnbull DM, Howell N: Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA. Nat Genet. 1999, 23: 147-View ArticlePubMedGoogle Scholar
- Carracedo A, Bär W, Lincoln P, Mayr W, Morling N, Olaisen B, Schneider P, Budowle B, Brinkmann B, Gill P, Holland M, Tully G, Wilson M: DNA Commission of the International Society for Forensic Genetics: guidelines for mitochondrial DNA typing. Forensic Sci Int. 2000, 110: 79-85.View ArticlePubMedGoogle Scholar
- Tully G, Bär W, Brinkmann B, Carracedo A, Gill P, Morling N, Parson W, Schneider P: Considerations by the European DNA profiling (EDNAP) group on the working practices, nomenclature and interpretation of mitochondrial DNA profiles. Forensic Sci Int. 2001, 124: 83-91.View ArticlePubMedGoogle Scholar
- Bandelt HJ, Parson W: Consistent treatment of length variants in the human mtDNA control region: a reappraisal. Int J Legal Med. 2007, 122: 11-21.View ArticlePubMedGoogle Scholar
- Steinlechner M, Parson W: Automation and high through-put for a DNA database laboratory: development of a laboratory information management system. Croat Med J. 2001, 42: 252-255.PubMedGoogle Scholar
- Brandstätter A, Niederstätter H, Pavlic M, Grubwieser P, Parson W: Generating population data for the EMPOP database – an overview of the mtDNA sequencing and data evaluation processes considering 273 Austrian control region sequences as example. Forensic Sci Int. 2007, 166: 164-175.View ArticlePubMedGoogle Scholar
- van Oven M, Kayser M: Updated comprehensive phylogenetic tree of global human mitochondrial DNA variation. Hum Mutat. 2008, 1039: e386-e394.Google Scholar
- Achilli A, Rengo C, Magri C, Battaglia V, Olivieri A, Scozzari R, Cruciani F, Zeviani M, Briem E, Carelli V, Moral P, Dugoujon JM, Roostalu U, Loogväli EL, Kivisild T, Bandelt HJ, Richards M, Villems R, Santachiara-Benerecetti AS, Semino O, Torroni A: The molecular dissection of mtDNA haplogroup H confirms that the Franco-Cantabrian glacial refuge was a major source for the European gene pool. Am J Hum Genet. 2004, 75: 910-918.PubMed CentralView ArticlePubMedGoogle Scholar
- Roostalu U, Kutuev I, Loogväli EL, Metspalu E, Tambets K, Reidla M, Khusnutdinova EK, Usanga E, Kivisild T, Villems R: Origin and expansion of haplogroup H, the dominant human mitochondrial DNA lineage in west eurasia: the near eastern and caucasian perspective. Mol Biol Evol. 2007, 24: 436-448.View ArticlePubMedGoogle Scholar
- Wallace DC: A mitochondrial paradigm of metabolic and degenerative diseases, aging, and cancer: a dawn for evolutionary medicine. Annu Rev Genet. 2005, 39: 359-407.PubMed CentralView ArticlePubMedGoogle Scholar
- Santos C, Martínez M, Lima M, Hao YJ, Simoes N, Montiel R: Mitochondrial DNA mutations in cancer: a review. Curr Top Med Chem. 2008, 8: 1351-1366.View ArticlePubMedGoogle Scholar
- EMPOP release 1. 2006, [http://www.empop.org]
- Brandstätter A, Zimmermann B, Wagner J, Göbel T, Röck AW, Salas A, Carracedo A, Parson W: Timing and deciphering mitochondrial DNA macro-haplogroup R0 variability in Central Europe and Middle East. BMC Evol Biol. 2008, 8: 191-PubMed CentralView ArticlePubMedGoogle Scholar
- Brandstätter A, Salas A, Niederstätter H, Gassner C, Carracedo A, Parson W: Dissection of mitochondrial superhaplogroup H using coding region SNPs. Electrophoresis. 2006, 27: 2541-2550.View ArticlePubMedGoogle Scholar
- Quintana-Murci L, Quach H, Harmant C, Luca F, Massonnet B, Patin E, Sica L, Mouguiama-Daouda P, Comas D, Tzur S, Balanovsky O, Kidd KK, Kidd JR, Veen van der L, Hombert JM, Gessain A, Verdu P, Froment A, Bahuchet S, Heyer E, Dausset J, Salas A, Behar DM: Maternal traces of deep common ancestry and asymmetric gene flow between Pygmy hunter-gatherers and Bantu-speaking farmers. Proc Natl Acad Sci USA. 2008, 105: 1596-1601.PubMed CentralView ArticlePubMedGoogle Scholar
- Behar DM, Villems R, Soodyall H, Blue-Smith J, Pereira L, Metspalu E, Scozzari R, Makkan H, Tzur S, Comas D, Bertranpetit J, Quintana-Murci L, Tyler-Smith C, Wells RS, Rosset S, the Genographic Consortium: The dawn of human matrilineal diversity. Am J Hum Genet. 2008, 82: 1130-1140.PubMed CentralView ArticlePubMedGoogle Scholar
- Sacconi S, Salviati L, Nishigaki Y, Walker WF, Hernandez-Rosa E, Trevisson E, Delplace S, Desnuelle C, Shanske S, Hirano M, Schon EA, Bonilla E, De Vivo DC, DiMauro S, Davidson MM: A functionally dominant mitochondrial DNA mutation. Hum Mol Genet. 2008, 17: 1814-1820.PubMed CentralView ArticlePubMedGoogle Scholar
- Kornblum C, Zsurka G, Wiesner RJ, Schröder R, Kunz WS: Concerted action of two novel tRNA mtDNA point mutations in chronic progressive external ophthalmoplegia. Biosci Rep. 2008, 28: 89-96.View ArticlePubMedGoogle Scholar
- Wang CY, Wang HW, Yao YG, Kong QP, Zhang YP: Somatic mutations of mitochondrial genome in early stage breast cancer. Int J Cancer. 2007, 121: 1253-1256.View ArticlePubMedGoogle Scholar
- Brandstätter A, Parson W: Mitochondrial DNA heteroplasmy or artefacts – a matter of the amplification strategy?. Int J Legal Med. 2003, 117: 180-184.View ArticlePubMedGoogle Scholar
- Ivanov PL, Wadhams MJ, Roby RK, Holland MM, Weedn VW, Parsons TJ: Mitochondrial DNA sequence heteroplasmy in the Grand Duke of Russia Georgij Romanov establishes the authenticity of the remains of Tsar Nicholas II. Nat Genet. 1996, 12: 417-420.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.